Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
The correct answer is option b. gonadotropin-releasing hormone.
Explanation:
The GnRH or the gonadotropin-releasing hormone is a releasing hormone that releases from the neurons of the hypothalamus. This releasing hormone is responsible for the secretion of the luteinizing hormone and follicle-stimulating hormone.
GnRH regulates both gonadotropin hormones, in which, LH (luteinizing hormone) responsible for the regulation of the male reproductive system and FSH (follicle-stimulating hormone) responsible for the female reproductive system and sperm production in testes in males.
Thus, the correct answer is option B. gonadotropin-releasing hormone.
Answer:
This question lacks options, options are:
A) ATP
B) NADP
C) Pryuvate
D) glucose
E) acetyl-CoA.
The correct answer is C) Pyruvate.
Explanation:
Pyruvate is a very important compound for the cell since it is a key substrate for energy production and glucose synthesis (neo-glycogenesis), that is, pyruvate is the end product of glucose breakdown in glycolysis. Before entering the mitochondria, it can be converted to lactate, through an anaerobic reaction (in the absence or under oxygen supply) of low performance in energy production, when the main pathway is interfered with. It can also be converted to the amino acid alanine. Within the mitochondria, it can be transformed, by pyruvate dehydrogenase (PDH), into acetyl-coenzyme A (acetyl-CoA), the entry point (substrate) of the Krebs cycle. In addition, by means of pyruvate carboxylase, it can be transformed into oxalacetate, which constitutes the first step in neoglycogenesis.
Answer:
Cell theory
Explanation:
Cell theory is a universal theory proposed by three scientists namely: Theodor Schwann, Mathias Schleiden and Rudolf Virchow in the 1830's. The cell theory compose of three components:
- All living things are made up of one or more cells.
- Cells are the basic and fundamental unit of life
- All cells emanate from pre-existing cells.
Based on this question, these three components are what makes up the CELL THEORY.
Cell reproduction is MOST applicable to growing and developing in organisms. Hope this helps!
Answer: GROW and DEVELOP
