Answer:
The smallest size tube that will hold the entire reaction is 0.5ml
Explanation:
25ul (DNA sample) + 25ul (enzyme) + 50ul (buffer) + 400ul (water) = 500ul total
since 1000ul=1ml, then 500ul = 0.5ml
Answer:
The device that mostlikely would not be used P20
Explanation:
P20 is a pipette used to measure and sample volumes of 20ul maximum.
Answer:
The reaction mix will have 400 units of the enzyme.
The working dilution will be 0.8 Units/ul.
Explanation:
The mix has 20ul of the enzyme which is 20Units/ul. Then we have 20ulx20Units/ul= 400 Units of enzyme in the mix.
Since the final volume is 500ul, the final concentration 400Units/500ul=0.8 Units/ul.
Answer:
The correct answer is option D.
Explanation:
In the presence of SDS single band appeared, while in the absence of SDS two bands appeared. SDS or sodium dodecyl sulfate refers to an anionic detergent, which combines with amino acid side chains providing the protein a net negative charge. It dissociates the non-covalent bonds.
In SDS-PAGE, the separation of proteins takes place on the basis of their molecular weight. Options A and B are incorrect as only in the presence of SDS, the separation of protein subunits takes place. Option C is also incorrect as a protein containing distinct molecular weight cannot show single band.
Option D is correct as the presence of SDS supplements a bunch of negative charges to the protein, thus, charge is not the factor. Therefore, the proteins are distinguished on the basis of the molecular weight. Thus, identical molecular weight demonstrates a single band. In the non-presence of SDS, charge performs a function along with the molecular weight, therefore, two bands appear.
Answer:
The main dangers worldwide are population growth and resource consumption, climate change and global warming, habitat conversion and urbanisation, invasive alien species, over-exploitation of natural resources and environmental degradation.
Explanation:
Answer:
Forestal tiene una cierta capacidad de "cicatrizarse" para recuperarse. Esta resiliencia ecológica varía con el contexto biogeográfico e histórico. El oeste de Nueva Orleans, seis meses después del paso del huracán Katrina
Explanation:
Answer:
Although there are many specific types of energy, the two major forms are Kinetic Energy and Potential Energy. Kinetic energy is the energy in moving objects or mass. Examples include mechanical energy, electrical energy etc.