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Fofino [41]
3 years ago
11

A culture of insulin-responsive cells is analyzed after stimulation with insulin. The insulin receptors are then isolated (assum

e that during the isolation protocol the insulin is removed from the receptor). Half of the sample is analyzed by SDS-PAGE followed by Coomassie blue staining. The other half of the sample is analyzed via SDS-PAGE followed by a Western blot. The primary antibody used in the blot recognizes phosphorylated tyrosine residues. Predict how many bands will be seen on the SDS-PAGE compared with the Western blot and explain your answer.
Biology
1 answer:
djyliett [7]3 years ago
4 0

Answer:

The α and β subunits of the insulin receptor form two bands in the SDS-PAGE gel. Moreover, in the Western blot analysis, there is just one band corresponding to the β subunit, since this subunit contains phosphorylated tyrosines, while α subunit doesn't have.

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<h2>Answer:</h2>

This can be explained with an example.

Let’s lake an example of two species 1 and 2.

Species 1 has a wide genetic diversity due to which organism are also diverse in phenotype. Their phenotype are:  

Black, dark brown, brown, dark red, red, light red, pink, skin color.

From left to right organism are becoming less adapted to high temperature relatively.

For species 2:  This species has less genetic diversity and its phenotypic diversity is as follow:

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From left to right organism are becoming less adapted to high temperature relatively.

Now if there is the sudden high temperature in the environment; 4 Types of phenotype will survive are black, dark brown, brown, dark red.

While there will only two types of organism in specie 2 which will survive are black, red.

So that’s how the diversity of organisms increase the chances that some will survive a major change in environment.


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