1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Fofino [41]
3 years ago
11

A culture of insulin-responsive cells is analyzed after stimulation with insulin. The insulin receptors are then isolated (assum

e that during the isolation protocol the insulin is removed from the receptor). Half of the sample is analyzed by SDS-PAGE followed by Coomassie blue staining. The other half of the sample is analyzed via SDS-PAGE followed by a Western blot. The primary antibody used in the blot recognizes phosphorylated tyrosine residues. Predict how many bands will be seen on the SDS-PAGE compared with the Western blot and explain your answer.
Biology
1 answer:
djyliett [7]3 years ago
4 0

Answer:

The α and β subunits of the insulin receptor form two bands in the SDS-PAGE gel. Moreover, in the Western blot analysis, there is just one band corresponding to the β subunit, since this subunit contains phosphorylated tyrosines, while α subunit doesn't have.

You might be interested in
Why should deepwater shrimp on different sides of the isthmus have diverged from each other earlier than shallow-water shrimp?
Nataly_w [17]

Answer:

Reproductive isolation occurs faster in deep-water shrimp than shallow-water shrimp.

Explanation:

Though in the same territory, the blockage caused by the isthmus would quickly and permanently isolate shrimps living in the deeper parts of the water, thus making them unable to breed. This situation would then caused lack of gene flow within the deep-water shrimps , and the emergence of new species that are genetically different (diverge) from one another

The shallow-water shrimp, on the other hand, experience minimal isolation due to the shallowness of water, and could still breed with one another. Thus, they experience a relatively lower reproductive isolation

6 0
3 years ago
Read 2 more answers
HELP PLEASE!!! How are cellular respiration and photosynthesis similar?
ale4655 [162]

Answer:

Your answer should be C I believe

4 0
3 years ago
Read 2 more answers
When you study heredity, you need to be able to compare generations. In this lab, you will compare different generations of mice
coldgirl [10]

first generation maybe im not a big biology freak and the second might be second generation im not super sure but srry if its not but its worth the shot :p

3 0
3 years ago
The________are part of the excretory system and are responsible for removing waste from the blood.
Lerok [7]
I believe Tubular Secretion

5 0
2 years ago
Describe in your own words what cell respiration is and why it is needs
valentina_108 [34]
<h2>Answer and Explanation </h2>

The cell respiration is a process in which the sugar molecules in the human body are broken into a form of energy which can be utilized by the cell. The cell respiration is needed to build up the adenosine triphosphate (ATP) and then it releases waste products from the cell. For this biochemical process, the cell uses oxygen hence, this process is also called aerobic respiration.

5 0
3 years ago
Other questions:
  • Below write a photo caption that compares the ecosystem shown above and the ecosystem shown on the previous page
    13·1 answer
  • Why don’t we have a complete fossil record of all life that’s ever lived?
    11·2 answers
  • How is it possible that over 20 million living organisms on the planet are made up of only 5 types of nucleotide based arranged
    5·1 answer
  • Crossing over occurs between:
    6·1 answer
  • Which of the following is Not noted when samples of insects are taken?
    9·1 answer
  • Drag each label to the correct location.<br> Classify the organisms based on what they eat.
    13·1 answer
  • Why are DNA fingerprints are more reliable than hand fingerprints in solving crimes.
    13·1 answer
  • 2. Which is the best definition of reader's theater?
    9·1 answer
  • PLSS HELP ME!! I WILL GIVE YOU BRAINLYEST!!
    8·1 answer
  • A duplicated chromosome consists of two ______. A duplicated chromosome consists of two ______. genomes centrosomes centromeres
    9·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!