Question

You are performing an in vitro experiment in which you will expose a material you are considering for a medical device to synovial fluid, which contains the proteins albumin, transferrin, and IgM at concentrations of 5, 0.5, and 0.05 mg/ml, respectively. Each of these components has a particular affinity for your material, with IgM being the highest and albumin being the lowest.

Answer 1

Synovial fluid

Explanation:

Due to the Vroman effect, albumin will initially attach and eventually be replaced by the IgM, which has a higher affinity for the material

The higher concentration of the albumin results in a greater initial surface concentration via diffusion, but it will eventually be displaced by the proteins with greater surface affinity (first the transferrin, and finally the IgM)

If more addition of albumin occurs, which has less affinity for the material surface, will have minimal effect if IgM is already adsorbed to material surface