The Cheshire cat caused the Queen of hearts to fall over
Answer:
Each mutant would be mated to wild type and to every other mutant to create diploid strains. The diploids would be assayed for growth at permissive and restrictive temperature. Diploids formed by mating a mutant to a wild type that can grow at restrictive temperatures identify the mutation as recessive. Only recessive mutations can be studied using complementation analysis. Diploids formed by mating two recessive mutants identify mutations in the same gene if the diploid cannot grow at restrictive temperature (non-complementation), and they identify mutations in different genes if the diploids can grow at restrictive temperature (complementation).
Explanation:
Recessive mutations are those whose phenotypic effects are only visible in homo-zygous individuals. Moreover, a complementation test is a genetic technique used to determine if two different mutations associated with a phenotype colocalize in the same <em>locus</em> (i.e., they are alleles of the same gene) or affect two different <em>loci</em>. In diploid (2n) organisms, this test is performed by crossing two homo-zygous recessive mutants and then observing whether offspring have the wild-type phenotype. When two different recessive mutations localize in different <em>loci</em>, they can be considered as 'complementary' since the heterozygote condition may rescue the function lost in homo-zygous recessive mutants. In consequence, when two recessive mutations are combined in the same genetic background (i.e., in the same individual) and they produce the same phenotype, it is possible to determine that both mutations are alleles of the same gene/<em>locus</em>.
Answer:
This question is incomplete as the term to match with the level of organisation is not included, the terms are;
circulatory system
cardiac muscle
heart
human body
The ANSWER is:
Organ = heart
Organ system = circulatory system
Tissue: cardiac muscle
Explanation:
The level of organization of multicellular organisms is made up of cell, tissue, organ, organ system and eventually organism.
- The tissue is composed of several cell, which are basic units of any living organism. Cells that perform similar function come together to form the tissue. Example is the CARDIAC MUSCLE in this question, which is a muscular tissue made up of cells called myocardiocytes.
- Organs are structures formed as a result of collection of tissues with similar function. For example, the HEART is a circulatory organ made up of cardiac tissues, connective tissues etc.
- Organ systems is made up of organs that perform the same function in a living organism. In the case of the CIRCULATORY SYSTEM, it is made up of organs such as heart, blood vessels, lungs etc.
