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Topoisomerase is the enzyme that can be added to correct the untwisting that is led to strain ahead of the replication fork.
Answer:
Glycolysis takes place in the cytosol which is part of the cytoplasm of the cell. Glucose is rearranged and phosphorylated to form F-1,6BP(fructose 16 bisphosphate which is very unstable) its splits into 2 phosphorylated 3C sugars (DHAP and G3P)(can interconvert) both form G3P to enter 3rd phase, G3P converted into pyruvate creates 4 ATP in total but has a net of 2 ATP and creates NADH. The raw materials needed are two molecules of NAD+ per glucose as well as 2 ATPs. Hope this helps!
Explanation: