The asthenosphere lies 80-200km below the surface under the lithosphere. Convection also occurs in the asthenosphere. The asthenosphere is mostly made of up rock material (magnesium and iron silicates). The asthenosphere makes up 6% of the mantle and lets the lithosphere move. 
Best of Luck!
 
        
             
        
        
        
Hey there,
Q1 & Q2) 
1) Heredity- Genes carry genetic information for cholesterol. So, it can be passed down from your parents.
2) Diet- Cholesterol depends on the food you eat. If you eat food with too much saturated fat, you get a high level of cholesterol. 
3) Weight- If you are obese, you are more prone to get cholesterol. Thus, you need to lose weight
4) Exercise- Exercise regularly to maintain a perfect cholesterol level
5) Stress- If you are a student, take breaks in between study timings to keep you less stress or if you are an adult, go for walks and do something that you like the most to calm your brain down.
Q3) Cholesterol causes plague to grow in your hearts. This thick, hard plague will block the arteries and will cause heart attacks and strokes. 
Q4) Pros- <span>Helps maintain healthy cholesterol levels, lowers risks of heart attacks and strokes
       Cons- C</span><span>ould create too many HDL leaving not enough cholesterol for the body to be healthy.
Hope this helps :))
~Top
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When it comes to population evolution and genetics, we cannot fail to cite the Hardy-Weinberg principle which emphasizes that if evolutionary factors such as natural selection, mutation, migration and genetic oscillation do not act on a particular population, the frequencies genotypic proportions will remain constant.
The five requirements for a population to be in Hardy-Weinberg equilibrium are:
- Large-scale breeding population: For a population to be in Hardy-Weinberg equilibrium, it is important that this population is large, as small populations favor genetic drift (unanticipated fluctuations in allele frequencies from one generation to another).
- Random mating: In order for the Hardy-Weinberg equilibrium to occur, it is necessary that the mating occur at random, with no preference for certain groups within the population. In this case, we say that the population is in panmixia, that is, they all mate at random.
- No mutations: Mutations alter the total alleles present in a population (gene pool). Therefore, in a Hardy-Weinberg equilibrium population, no mutations should occur.
- No gene flow: When there is gene flow due to migration or immigration of individuals, some genes may be included or excluded from the population. Thus, in an equilibrium situation, no gene flow occurs.
- Lack of natural selection: For a population to be in Hardy-Weinberg equilibrium, natural selection must not be acting on it. If natural selection acts, some genotypes will be selected, modifying the allelic frequencies of the population.
 
        
             
        
        
        
Answer:
-use PCR to amplify the gene for GFP
-perform a restriction digestion of the GFP gene and the plasmid
-ligate together the GFP gene and the plasmid to generate a recombinant plasmid
-transform bacteria with the recombinant plasmid using electroporation
-plate the bacterial cells, and screen for positive transformants