I would love to help answer, but i think i need a little more context here.
Answer:
The student forgot to add reverse transcriptase
Explanation:
Reverse transcriptase is an enzyme, also called DNA polymerase or RNA-dependent, responsible for carrying out the transcription process, which corresponds to an “inverted copy”, as opposed to the cellular pattern. In other words, this enzyme polymerizes DNA molecules from RNA molecules, the opposite of what commonly occurs in cells.
The student, exposed in the question, wanted to create cDNA through an isolated and amplified mRNA sample, but this student did not use reverse transcriptase, so it would be impossible for his experiment to have good results.
