The synthesis<span> of </span>proteins<span> takes two steps: transcription and translation. Transcription takes the information encoded in DNA and encodes it into mRNA, which heads out of the cell's nucleus and into the cytoplasm. During translation, the mRNA works with a ribosome and tRNA to </span>synthesize proteins<span>.</span>
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
b- It is a spontaneous process.
Explanation:
Passive transport is the type of transport of substances across the plasma membrane that occurs without energy expenditure and occurs spontaneously. There is no energy expenditure during passive transport because substances naturally move from the more concentrated to the less concentrated medium, ie in favor of the concentration gradient.
Substance transport occurs until concentrations inside and outside the cell are equalized.
According to this conceptualization we can conclude that the correct answer to your question is the letter B.
Element
An element is a pure substance that cannot be broken down into other substances, an element is made up of only one type of atom.
Hope this helps :)
Answer:
It is True
Explanation
They thrive at temperatures in the 41 to 140 degrees.
