1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
GaryK [48]
3 years ago
9

Suppose that a single protein is significantly overexpressed in one of two different cell lines. The proteins are identical, but

Northern blot analysis indicates that the mRNA for the protein in the overexpressing cell line is about 200 base pairs shorter. Subsequent reverse transcriptase PCR has revealed that the truncation occurs in the 3ʹ untranslated region. How might this lead to overexpression of the protein?
Biology
1 answer:
mart [117]3 years ago
7 0

Explanation:

Many mRNAs have sequences of 50 - 150 nt rich in A and U in the 3' untranslated region. These sequences, called AU-rich elements, are important to regulate mRNA stability, because they bind proteins that target the mRNA for degradation.

When a protein binds to the AU-rich element of the mRNA, the polyA tail can be shortened and/or the 5'cap is removed, promoting mRNA degradation. In addition, the AU-rich elements in some mRNAs have been shown to inhibit protein translation.

The mRNA of the overexpressing cell line is about 200nt shorter than the normal cell line; this could mean that it has a deletion in the 3'UTR comprising an AU-rich element. If this is missing, the mRNA will have an extended half-life (because it won't be targeted for degradation) or it will be translated more. As a result, the protein expression will be increased.

You might be interested in
Complete the sentence: Ecosystem distribution
Step2247 [10]

Answer:

<em>Hypotheses may vary. Most students will probably state that ecosystems at </em>

<em>high altitudes will support little vegetation. </em>

Explanation:

Ecosystem distribution is different at different places. Regions at high elevations might usually have low varieties of plants and animals but it cannot be true for every ecosystem located as higher altitudes.

Some communities might be well adapted to live in the cold and hence progress rapidly.

3 0
3 years ago
The concentration of potassium is higher in red
dimaraw [331]
<span>(4) active transport
</span>Active transport is the movement of molecules across a cell membrane in the direction against their concentration gradient, i.e. moving from an area of lower concentration to an area of higher concentration.
6 0
2 years ago
does a glucose come out of the body through faces or urine, or is it utilized inside the body and disappears.?
Georgia [21]

Answer:

Yes, glucose expel out of the body through urine, if the concentration of glucose is high in blood.

Explanation:

Glucose is monosaccharide which is used by the body for the production of energy in the form of Adenine tri phosphate. This glucose is stored by the body in the form of glycogen when its concentration is high. If glucose level is very high than optimal level in the blood so the kidney send it to the urine for the removal from blood.

3 0
3 years ago
Read 2 more answers
The meningeal branch of a spinal nerve actually reenters the vertebral canal to innervate the meninges and blood vessels. The me
Shalnov [3]

Answer:

True

Explanation:

Spinal nerve divides into several branches after passing through its intervertebral foramen. These branches are called rami. A spinal nerve gives off several branches such as anterior ramus, posterior ramus, rami communicantes, and a meningeal branch. The meningeal branch reenters the vertebral cavity through the intervertebral foramen. It serves to supply the vertebrae, vertebral ligaments as well as the blood vessels of the spinal cord, and meninges.

4 0
3 years ago
1, describe how thermal energy/heat affects atoms within the 3 state of matter.
Arisa [49]

Answer:

When heat is added to a substance, the molecules and atoms vibrate faster. As atoms vibrate faster, the space between atoms increases. The motion and spacing of the particles determines the state of matter of the substance. ... Solids, liquids and gases all expand when heat is added.

Explanation:

4 0
3 years ago
Other questions:
  • Select the correct statement about plant life cycles.
    14·1 answer
  • What part of the brain stores stressful experiences as negative emotional memories?
    12·1 answer
  • What is the outcome when a cell undergoes meiosis
    15·2 answers
  • In which phase of the eukaryotic cell cycle do microtubules arrange in preparation for mitosis? answer cytokinesis, g1 phase, g2
    15·1 answer
  • Describe how changes in limiting factors can impact population growth.<br><br> Need help asap!!!
    15·1 answer
  • Darwin observed that finches on the Galapagos Islands have different kinds of beaks. Which conclusion is best supported by his o
    10·2 answers
  • How many groups are in the modem periodic table
    13·1 answer
  • How does the nucleus help the cell process waste?
    12·1 answer
  • What is the chemical reaction mechanism by which cells make polymers from monomers?
    15·1 answer
  • Which is an example of a decomposer?<br> A a hawk<br> B a mouse<br> C a flower<br> D a mushroom
    11·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!