Which of the statements best describes the way you would engineer bacterial cells to produce a human protein?A. One restriction
enzyme would be used to cut the donor human DNA and a different one would be used to cleave the vector DNA. B. One restriction enzyme would be used only to cleave the vector DNA. C. One restriction enzyme would be used only to cleave the donor human DNA. D. The same restriction enzyme would be used to cut both the donor human DNA and the vector DNA
The correct answer is option D. The same restriction enzyme would be used to cut both the donor human DNA and the vector DNA.
Explanation:
The way one could engineer bacterial cells to produce a human protein is by allowing bacteria to taking up the recombinant DNA. This recombinant DNA are inserted in the form of the vectors.
Recombinant DNA technology or RDT allows individual fragments of DNA from any donor human genome to be inserted into vector DNA molecules with the help of the specific restriction enzyme for both vector DNA and donor DNA.
Thus, the correct answer is option D. The same restriction enzyme would be used to cut both the donor human DNA and the vector DNA.
