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Angelina_Jolie [31]

3 years ago

15

Which of the following is a characteristic of a prokaryotic cell? It does not have a nucleus. It has membrane-bound organelles.

It is larger in size than a eukaryotic cell. It is not present in unicellular organisms.

1 answer:

Dima020 [189]3 years ago

8 0

The answer is, "It has membrane-bound organelles."

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Put the steps to produce spider silk protein in yeast in the correct order. 1 Purify protein. 2 Join yeast regulatory sequence a

Anuta_ua [19.1K]

<h2>The correct sequence is: 4>2>3>5>1.</h2>

Explanation:

The given question shows the sequence in which silk encoding gene from spider can be expressed in yeast in order to produce Recombinant Silk.
Originally, silk can be obtained from spider. But for the commercial production and meeting the high demand of silk it is not economical to depend on spider to produce silk as they not only take long time to produce silk them but also they have a longer life-cycle and longer generation time as a result of which the quantity of silk obtained by spider per generation is very low. Also, spiders require specialized habitat and complex nutrient for their survival.
Hence, yeast, an unicellular eukaryote, which has a shorter generation time and simple nutrient requirement for its survival, can be used for the commercial production of silk.
For this, initially a yeast gene which is constitutively expressed in yeast is selected and the coding region of this gene is removed with the help of enzymes, keeping the regulatory sequences like the promoter, enhancer, terminator, etc, intact in the 5' and 3' region of the gene.
A constitutive gene is the one which is expressed continuously at large amounts. This will result in the increased synthesis of the recombinant silk protein.
Next, the coding sequence of the gene encoding for silk protein which is obtained from the spider is joined with the help of enzymes to the regulatory gene sequences of the yeast which was obtained in the first step.
Next, this recombinant gene comprising of the yeast regulatory sequence and the coding region of the gene encoding for silk obtained from spider is inserted into the yeast cells.
Next, the yeast cells are allowed to express the silk coding gene by the process of transciption and translation utilizing the yeast specific RNA (Ribonucleic acid) polymerase (recognizes the yeast-specific promoter and enhancer in the recombinant gene) and yeast-specific ribosome, respectively.
The recombinant silk protein produced in the yeast can then be subjected to purification.

5 0

4 years ago

In a wave, what is a distance between two crests or two troughs called?

masha68 [24]

Answer:

the distance between 2 crests & 2 troughs is known as wavelength

Explanation:

4 0

3 years ago

In exfoliative cytology, epithelial cells are removed and examined without any lasting damage to the tissue from which these cel

torisob [31]

Because epithelial tissue is capable of regeneration.

The skin consists of two tissues: the outermost, the epidermis, resting on a vascularized connective tissue, the dermis. In the epidermis, which includes several layers of cells, the outer layer is formed of dead cells that are desquamating and are constantly being replaced by proliferating basal cell cells. So, under normal physiological conditions, the epidermis, like all the epithelia, is in constant renewal. On the other hand, when accidentally, the upper parts of the epidermis are damaged as a result, for example, of a burn or a slight abrasion, the destroyed portion is regenerated thanks to an accelerated proliferation of basal epidermal cells.

4 0

3 years ago

Read 2 more answers

If brick, dry soil, paper and water were exposed to sunlight for the same amount of time, which substance would heat up the fast

SSSSS [86.1K]

Water - It has the highest specific heat and therefore is the substance that would heat up the slowest.

3 0

4 years ago

Facilitated diffusion and active transport are different in that _____

goblinko [34]

Answer:

facilitated diffusion doesn't require energy, while active transport requires energy. Facilitaded difusion happens down the concentration gradient (molecules move from area of higher concentration to area of lower concentration), while active transports happens up the concentration gradient (from lower concentration to higher concentration).

Explanation:

5 0

3 years ago