It is unlikely the membrane would become depolarized because although Na+ is coming in, K+ would be leaving simultaneously (canceling each other out). I hope my answer has come to your help. God bless and have a nice day ahead! Feel free to ask more questions.
Explanation:
Please post a picture of the question, since there is no way to match anything without more information. :)
Answer:
Use a step wise process
Explanation:
In order to find the presence of the recombinant protein:
- Culture the cells that you believe harbors the plasmid for the recombination protein (+) and also cells that do not (-). This is your control.
- Extract the plasmid from both strains.
- Observe the plasmid map and the area of your insert. Use this to select the restriction enzymes at the beginning and the end of the YFG gene. You can also use a restriction site inside the gene but not in the plasmid for better control.
- Perform the restriction enzyme digest on both (+) and (-) according to your protocol and separate using gel electrophoresis.
- Observe the separate bands that you see. In the (+) there should be a band that is the size of your insert and a larger band that is the size of your plasmid control band and in the (-) there should be a single band that is the size of the plasmid but non the size of the insert.
Answer:
A. Uniformitarianism
Explanation:
The principle of Uniformitarianism was proposed by a Scottish Geologist, James Hutton.
The principle is simply stated as "the present is the key to the past".
The processes that have occurred in geologic past is still in play today. By looking at rock sequences in our present day, we can actually have a firm grasp of the processes they have undergone in the past.
In aluminium fluoride, aluminium carries positive charges so fluoride should carry negative charge to be electrically neutral.So for aluminium ion with three positive charges fluoride ion should possess three negative charges to make aluminium fluoride electrically neutral.
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