<h2>Functions of spleen</h2>
Explanation:
The major functions of spleen besides filtering the blood of foreign materials and phagocytosis of old defective erythrocytes are:
- Initiate immune responses against infections by liberation of antigen and activation of lymphocytes (T and B cells)
- Secretes bactericidal agents which act against invading bacteria
- Secretes interleukins which promote the growth of lymphocytes or antibodies that act against pathogens
- Reservoir: Pools blood by storing thrombocytes, immature erythrocytes.
- Hematopoiesis: Blood cells like erythrocytes, lymphocytes and monocytes develop and mature in spleen especially during the early fetal life.
Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid. The most common Type II enzymes are those like HhaI (NEB #R0139), HindIII (NEB #R0104), and NotI (NEB #R0189)
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