Answer:
sexual
Explanation:
there are two types of reproduction,
the boring kind and the fun kind
Uh i mean asexual and sexual
in asexual the DNA is copied pretty much exactly the same so there is no room for improvement so if something suddenly changes the organism dies.
In sexual, The DNA of 2 different organisms are combined and this allows for greater variation in genetic evolution. so if something in the environment happens, the ones with a better genes will survive and reproduce more while the other ones die.
Scientific law. For example, Law of Universal Gravitation.
Answer:
2 molecules of ATP and 2 molecules of NADH
Explanation:
Glycolysis is the first step of cellular respiration (break down of glucose to extract energy) which occurs in the cytoplasm. Glycolysis is a pathway common to all living organisms- prokaryotes and eukaryotes, as it does not require oxygen to occur.
Glycolysis occurs in two major phases (ten steps) requiring 10 enzymes catalyzing each step; the energy-requiring phase and the energy-requiring phase.
In the energy-requiring phase, the starting molecule (glucose) gets rearranged in a series of chemical reactions, and two phosphate groups gets attached to it producing fructose-1,6-bisphosphate which is unstable, This modified sugar then splits in half due to its instability to form two different but inter-convertible phosphate-bearing three-carbon sugars (Dihydroxyacetonephosphate, DHAP and Glyceraldehyde-3-phosphate, G3P). Because the phosphates used in these steps come from 2 ATP molecules, 2 ATP molecules get used up in this phase
All the DHAP molecules get converted to G-3-P in order to enter the next phase.
In the energy-recovering phase, the 3-carbon sugar (G3P) is converted into another three-carbon molecule called pyruvate, through a series of reactions. In these reactions, two ATP and 1 NADH molecules are made. This recovery phase occurs twice (one for each of the two isomeric three-carbon sugars, DHAP and G3P). Hence, a total of 4 ATP and 2 NADH molecules are produced in this phase.
Overall, Glycolysis converts one glucose (six-carbon) molecule to two pyruvate (three-carbon) molecules and a net release of 2 ATP molecules (4 overall - 2 used) and 2 NADH molecules.
No somethings only have one cell. they are called unicellular organisms.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
