Question

The primary structures of GFP and BFP proteins each contain 238 amino acids. However, BFP substitutes two amino acids found in the GFP primary structure. Compare the location of the GFP band in “G-Boil” to the location of the BFP band in “B-Boil”. Did the difference in their primary structure result in a difference in their mobility in the gel? Explain why or why not.

Answer 1

Answer:

Yes they show difference in their mobility due to the difference in their molecular weight.

Explanation:

GFP is green fluorescent protein and BFP is blue fluorescent protein that are used in recent molecular techniques. Gel electrophoresis can be used to understand the molecular weight of protein based on their mobility.

The GFP and BFP have 238 amino acids but two amino acids have been substituted that found in the GFP primary structure. The GFP band in G-Boil and BFP band in B-Boil shows different location. The substitution of two amino acids reduces the molecular weight of the protein. The protein with less molecular weight shows high mobility in the gel.