In residential heating, convection is the mechanism by which heat is lost by warm air leaking to the outside when the doors are opened, or cold air leaking into the house through the cracks or openings in walls, windows, or doors. When cold air comes in contact with the heater in a room, it absorbs the heat and rises.
Answer:
I would say bias.
Explanation:
bias: prejudice in favour of or against one thing, person, or group compared with another, usually in a way considered to be unfair.
"there was evidence of bias against foreign applicants"
When you are being biased, you are putting your own opinion into what you are doing or you are trying to persuade the person hearing you, reading what you wrote.
Therefore, Evidence without bias increases its reliability.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
c
Explanation:
ccccccccccccccccccccccccc c
follow my I g
