Answer:
In the natural world, limiting factors like the availability of food, water, shelter and space can change animal and plant populations. Other limiting factors, like competition for resources, predation and disease can also impact populations.
Explanation:
Persipitation and geographic layout
Well assuming the original cell is a prokaryotic, you wouldn't be able to remove the nucleus because prokaryotic cells don't have a nucleus to begin with. if this doesn't help then: The cell would lack genetic information.