Explanation:
You can do two controls:
1) Check if the cells are viable by plating the transformed E.coli on LB medium with no antibiotics. If the cells are alive, a lawn will be seen, as there is no selection pressure. If the cells are dead, no colonies will be seen.
2) Check if the cells are competent by transforming them with a control plasmid. This is another plasmid, either commercial or already used in the lab, that contains the gene for resistance to kanamycin, and is known to work. If this positive control is used to transform the cells and still no growth is seen in the LB plates, then the E.coli are probably not competent.
The order is Hepatocyte, Bile canaliculus, Common hepatic duct, Cystic duct, and Gallbladder.
<h3>
What is bile?</h3>
The liver of most vertebrates produces bile, also known as gall, which is a dark-green to the yellowish-brown fluid that aids in the small intestine's breakdown of lipids. Bile is continuously created by the liver in humans (liver bile), and it is stored and concentrated in the gallbladder. Hepatic bile is made up of 200 meq/l inorganic salts, 0.7% bile salts, 0.2% bilirubin, 0.5% lipids (cholesterol, fatty acids, and lecithin), and 97-98% water. Biliverdin, a green oxidized version of bilirubin, is one of the two primary pigments in bile. They combine to give feces their specific brown hue color. Adult humans produce about 400 to 800 milliliters of bile every day.
To learn more about bile, visit:
brainly.com/question/16041873
#SPJ4
Answer:
tetracycline
Explanation:
A plasmid is a small, circular, double-stranded DNA molecule, which is physically separated from chromosomal DNA and is able to replicate independently. Moreover, plasmid transformation is the process by which exogenous DNA is introduced into a cell (usually a bacterial host cell). In this case, <em>Escherichia coli </em>K12 cells were transformed with the pBR322 plasmids that encode both ampicillin and tetracycline resistance genes. According to data, plasmids were firstly cleaved with the PstI type II restriction endonuclease within the ampicillin resistance gene, thereby these plasmids lose the ampicillin-resistance gene that allows selecting <em>E. coli </em>K12 cells by their resistance to ampicillin. In consequence, tetracycline is the only one selective marker that will indicate that the bacteria have incorporated the plasmid.