Question

A fusion protein can be sythesized to carry a specific tag, which is useful in identifying the proteins in various experiments. Fusion proteins with the FLAG epitope bind to specific anti-FLAG antibodies and carry this extra sequence: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. The bioteh company that sells anti-FLAG antibodies states that fusion proteins carrying the Flag epitope display the sequence on the surface of the protein. This is likely_______ because________of the eight residues are________, making FLAG highly_______and likely to interact______Al with water.

Answer 1

Answer:

A fusion protein can be sythesized to carry a specific tag, which is useful in identifying the proteins in various experiments. Fusion proteins with the FLAG epitope bind to specific anti-FLAG antibodies and carry this extra sequence: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. The bioteh company that sells anti-FLAG antibodies states that fusion proteins carrying the Flag epitope display the sequence on the surface of the protein. This is likely A short hydrophilic 8-amino acid peptide  because of its hydrophilic nature of the eight residues are Likely to be located on the surface of the fusion protein, making FLAG highly Specific and easily accessible for cleavage by enterokinase and likely to interact with other epitope and domains Al with water.

Explanation:

FLAG, a short hydrophilic peptide consisting of 8 amino acids have been widely used as a fusion tag for purification and detection of a wide variety of recombinant proteins. the 3× FLAG system is an improvement upon the original system by fusing 3 tandem FLAG epitopes (22amino acids). detection of fusion protein containing 3× FLAG is enhanced up to 200 times more than any other systems.