Confirm that the sample has both helicase and primase activities, but not the ability to synthesize DNA
- The replisome is a multiprotein-RNA complex that is required for the replication of DNA. The replisome consists of different proteins which include:
1. Helicase enzymes that unwind and separate the DNA strand
2. Replication factor C (RFC), a DNA-dependent ATP that acts as an activator of DNA polymerase.
3. Proliferating cell nuclear antigen (PCNA)
4. DNA gyrase/topoisomerase that cuts and reseals DNA strands, which is essential for DNA synthesis.
5. Primase enzyme that synthesizes short RNA fragments called primers.
6. DNA polymerase III, an enzyme that catalyzes the polymerization of deoxyribonucleotides into a DNA strand.
7. DNA ligases enzymes that form phosphodiester bonds at a single-strand break in DNA
- A primosome is a multiprotein complex responsible for creating RNA primers on single-stranded DNA (ssDNA) during the replication process.
- The primosome is composed of different primase and helicase enzymes. In<em> Escherichia coli</em>, it consists of PriA helicase, PriB, PriC, DnaB helicase, DnaG (encoded by the dnaG gene), DnaC and DnaT primase.
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i think the answer should be asymptomatic
Answer:
it is 180 million years old
Answer:
(a) When the enzyme phosphorylase is allowed to treat with the ATP and phosphorylase kinase, this helps in the activation of the more active form of the phosphorylase. This increase the process of the glycogen breakdown in the cell.
(b) PP1 ( protein phosphatase 1) removes the phosphate group from the substrate that results in the activation of the less active form of the enzyme. The process of glycogen breakdown in the cell will decrease.
(c) The hormone epinephrine causes the release of cAMP that increases the kinase activity. The active form of the enzyme predominates and increases the glycogen breakdown in the body.
