<span>3,3',5,5'-Tetramethylbenzidine or TMB is a chromogenic substrate visualising reagent used in enzyme-linked immunosorbent assays (ELISA), it yields a blue color when oxidized, typically as a result of oxygen radicals produced by the hydrolysis of hydrogen peroxide by HRP, (thermofisher). The expected colorimetric result from the negative control in your assay will be no development of blue color</span>
When you scrape your original sample onto your agar plate, you cannot see how much single bacteria or where the individual bacteria is on your plate- since it's invisible to the naked eye. But when the bacteria start to multiply, you start to see the individual colonies. (from the single bacteria, it begins to multiply within 20 min. maybe after 1-2 days you'll see a colony, meaning there are millions of bacteria)
for example, if you take a water sample and spray it onto an agar plate, you won't know which parts of the agar plate the bacteria landed on. however, when they start to multiply from a single bacterium, you'll see where each starting bacterium was because now you can see a whole bunch of bacteria. (remember that a colony contains millions of bacteria- which allow it to be visible to the naked eye).
so you count the number of colonies, and that'll tell you how much bacteria you started with. if you look at the size of the colonies, you're only looking at how long you allowed the bacteria to incubate (since from the single bacteria that you started with, it's only multiplying and growing outwards).
Exons are the DNA fragments that get to be transcripted and translated into proteins. Option b)<u> </u><u>Exons</u> end up being expressed in the protein.
<h3>What are introns and exons?</h3>
Even though more than 70% of the DNA is related to genes, just a part of the genes is translated to proteins. Most genes have DNA segments that are not transcripted or translated intercalated with segments that are translated.
- Introns are non-encoding sequences that produce interruptions in a eukaryotic gene.
- Exons are the encoding sequences. Exons are the nucleotidic sequences that do codify proteins.
Most of the eukaryotic, multicellular genes contain introns in their sequences, intercalated with exons.
Introns are transcripted to mRNA molecules and get excised before translation by a specific mechanism. This occurs before the mRNA leaves the nucleus. The introns are excised and the exons must splice to form a unique molecule. This process is known as splicing.
Exonic sequences are the fragments that are transcripted and translated into proteins.
The correct option is b). Exons end up being expressed in the protein
You can learn ore about exons at
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