Answer:
Corresponding mRNA Sequence: ALGUG GAACC_GCUG CLGA
Amino Acid Sequence: METHIONINE-LEUCINE-GLUTAMIC ACID-PROLINE
<span>3,3',5,5'-Tetramethylbenzidine or TMB is a chromogenic substrate visualising reagent used in enzyme-linked immunosorbent assays (ELISA), it yields a blue color when oxidized, typically as a result of oxygen radicals produced by the hydrolysis of hydrogen peroxide by HRP, (thermofisher). The expected colorimetric result from the negative control in your assay will be no development of blue color</span>
The steps are in google first tab search it
Prokaryotic has no nucleus "pro"= no
eukaryotic does have a nucleus
