<h2>Resistance to antibiotics </h2>
Explanation:
Bacteria are single-celled organisms usually found all over the inside and outside of our bodies
- Antibiotic resistance is the ability of bacteria or other microbes to resist the effects of an antibiotic
- Antibiotic resistance occurs when bacteria change in some way that reduces or eliminates the effectiveness of drugs, chemicals, or other agents designed to cure or prevent infections,the bacteria survive and continue to multiply causing more harm
- There are two main ways that bacterial cells can acquire antibiotic resistance,one is through mutations that occur in the DNA of the cell during replication and the other way that bacteria acquire resistance is through horizontal gene transfer
- Through the process of replication bacteria develop mutations that make them resistant to antibiotics
- Bacteria with the resistant mutation have a better chance of survival against antibiotics
- Resistant bacteria continue to multiply even when exposed to antibiotics
- In horizontal gene transfer,antibiotic resistant genetic material is transferred between different bacterial cells which can happen in three different ways: transformation,transduction or conjugation
Definition
A technique which is used to separate, DNA, RNA or protein pieces from each other under the influence of electric field on the basis of their molecular size is known as gel electrophoresis.
Explanation
This method is very reliable for separation of large size molecule (over 1 million Da). Materials which are required for gel electrophoresis include:
1. TAE stock buffer
2. 1% agrose gel
3. Nucleic acid loading dye
4. Ethidium bromide
Procedure:
First prepare a stock solution of TAE buffer by adding appropriate amount of TAE in distilled water. then prepare 1% agrose by adding 1X TAE, some amount of agrose in water and heating it in microwve oven to mix them will. then pour agrose gel on tray and fix comb in it and keep it untill agrose dry. Then remove comb and pour some quantity of nucleic acid along with loading dye and ethidium bromide in each well. EtBr is used for staining nucleic acid.
When sample is poured in all well also pour reference marker in one well for comparison. now connect it with voltage for 30-35 min. After this take gel and see it under UV. a large number of nucleic acid pieces will be seen on gel under UV. those pieces which have small molecular weight will cover more distance compared to those having larger molecular weight.
I think that the answer would be C
