All categories

3 years ago

Explain fully, in your own words, why the temperature during PCR goes through cycles of 94C, 50C, 72C.

Physics

1 answer:

Aliun [14]3 years ago

3 0

Answer:

Explanation:

The DNA polymerase used in PCR reaction usually can tolerate high temperature (94degree celcius), the temperature necessary to separate two complementary strands of DNA in a test tube.

If PCR goes through 72degree celcius, which is the temperature optimum for taq polymerase. It starts the extension process where taq polymerase will work away from the primer provided and generated a new DNA strand.

If PCR goes through 50degree celcius, it is annealing temperature. It is related to the melting temperature of the primers and must be determined for each primer pair used in PCR.

You might be interested in

Water from a vertical pipe emerges as a 10-cm-diameter cylinder and falls straight down 7.5 m into a bucket. The water exits the

cupoosta [38]

The diameter of the column of the water as it hits the bucket is 4.04 cm

The equation of continuity occurs in the fluid system and it asserts that the inflow and the outflow of the volume rate at the inlet and at the outlet of the system are equal.

By using the kinematics equation to determine the speed of the water in the bucket and applying the equation of continuity to estimate the diameter of the column, we have the following;

Using the kinematics equation:

$\mathbf{v_f ^2 = v_i^2 + 2gh}$

$\mathbf{v_f ^2 =(2.0)^2 + 2\times 9.8 \times 7.5}$

$\mathbf{v_f ^2 =151 m/s}$

$\mathbf{v_f =\sqrt{151 m/s}}$

$\mathbf{v_f =12.29 \ m/s}$

From the equation of continuity:

$\mathbf{A_iV_i = A_fV_f}$

$\mathbf{\pi r^2_iV_i = \pi r^2_fV_f}$

$\mathbf{ r^2_iV_i = r^2_fV_f}$

$\mathbf{ (\dfrac{10}{2})^2\times 2.0 = r_f^2 \times 12.29}$

$\mathbf{ 50 = 12.29 \times r_f^2}$

$\mathbf{ r_f= \sqrt{\dfrac{50}{12.29} }}$

$\mathbf{ V_f= 2.02 \ cm }$

Since diameter = 2r;

∴

The diameter of the column of the water is:

= 2(2.02) cm

= 4.04 cm

Learn more about the equation of continuity here:

brainly.com/question/10822213

6 0

2 years ago

Is it illegal to attempt gerrymandering

IRINA_888 [86]

Explanation:

The US Supreme Court has affirmed in Miller v. Johnson (1995) that racial gerrymandering is a violation of constitutional rights and upheld decisions against redistricting that is purposely devised based on race. However, the Supreme Court has struggled as to when partisan gerrymandering occurs (Vieth v.

8 0

3 years ago

Two cars are heading towards each other but are 12 km apart. one car is going 70 km/hr, and the other is going 50 km/hr. how muc

vova2212 [387]

<span>12-50t=70t, t= 0.1h = 6 minutes.</span>

3 0

3 years ago

At what height h above the ground does the projectile have a speed of 0.5v?

dedylja [7]

Answer:height above ground at which projectile have velocity

0.5v is (0.0375v^2)

Explanation: