is the average weather condition of an area over a long period of time
The three foremost forms of photosynthesis are C3, C4, and CAM (crassulacean acid metabolism)
C4 flowers are so-called because the primary manufactured from CO2 fixation is a C4 natural acid, oxaloacetate, shaped by way of the carboxylation of phosphoenolpyruvate (PEP) by way of PEP carboxylase. The oxaloacetate is converted to other C4 acids (malate or aspartate) and transferred to the packaging sheath.
Calvin cycle is the main pathway of carbon fixation in vegetation, algae, and cyanobacteria. The opportunity pathways of carbon fixation are the Reductive citric acid cycle in microorganisms. 3-hydroxypropionate cycle in bacteria and archaea.
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Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.