1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Aleksandr [31]
3 years ago
11

6. (3 pts) After transformation, 40 colonies were present on the plate. What is the transformation efficiency of the competent c

ells if 100 ng of DNA was used for transformation of 100 ul competent cells
Biology
1 answer:
natali 33 [55]3 years ago
6 0

Answer:

Given:

Number of colonies on plate = 40

Amount of DNA used for transformation = 100ng

Volume of competent cells used = 100ul

Total volume = 1ml (1000ul)

Volume plated for growth = 200ul

The amount of cells transformed per 1 µg of DNA is called the transformation efficiency

(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA

Amount of DNA used for plating = 100ng * (200/1000)

                                                      = 100ng * (1/5)

                                                      = 100/5 = 20ng

Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng

               (2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA

Transformation efficiency = 2000 Transformants / ug DNA

                                          = 2 x 10³ Transformants / ug DNA

Explanation:

Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.

You might be interested in
Which of the following is an example of an involuntary muscle? *
spayn [35]
B! Cardiac muscle hope it helps sorry if it doesn’t!
7 0
2 years ago
Which of the following is NOT a density-dependent factor affecting population growth?
sveta [45]

B

<u>Temperature</u> is NOT a density-dependent factor affecting population growth

Explanation:

Density-dependent factors mean variables that are affected by the density of the population. The opposite of density-depend is density-independent factors. These are those variables that affect the density of the population but are not affected by the density of the population.

In this case, temperatures are not affected by the density of the population. However, temperatures affect the density of the population. Most individuals can only survive within an optimal temperature range.

The rest of the choices are density-dependent variables

Learn More:

For more on dependent and independent variables check out;

brainly.com/question/3205171

brainly.com/question/3205171

brainly.com/question/4600039

#LearnWithBrainly

7 0
3 years ago
Which additional observation would support the conclusion that the deer population is not changing size from year to year, meani
gavmur [86]

Answer:

The presence of predators of deer which does not allow increase in the population of deer year after year.

Explanation:

In an forest ecosystem, one animal controls the population of another organisms and thus there is no increase in the population of that organism and the ecosystem is in equilibrium state. The population of deer did not increase due to the presence of predators such as lion, cheetah etc and the net population is 0.

7 0
3 years ago
Which Chemicals are major contributors to the Ozone layer destruction? Select TWO answer choices.
Romashka [77]

Answer:

The damage to ozone layer is done by halogens such as Chlorine and Bromine.

3 0
3 years ago
Read 2 more answers
A crop is treated with a pesticide and 98% of the insects are killed. what will most likely happen to the remaining insects?
Marina CMI [18]

The 2% of the insects will begin to reproduce and grow their population exponentially and in time enable the population to recover. This is if the population is large enough to enable random mating. The 98% reduction in population drastically reduces competition for the remaining individuals hence giving them abundant resources for them to thrive.   






3 0
3 years ago
Other questions:
  • Can someone help me with this question??
    9·1 answer
  • What is it’s first base?
    13·1 answer
  • Carbon-14 has a half life of 5,770 years. A fossil was found that had 1/32 of its original Carbon-14 remaining. How old is the f
    11·1 answer
  • Sickle-cell anemia is an example of codominance. What implications does this have for people with one or two copies of the sickl
    13·1 answer
  • Evaluate Models Cells are often compared to factories. How is a factory a useful model for explaining the cell?
    9·1 answer
  • Abbey always gets zits on her chin and she can't figure out
    8·2 answers
  • 1. A piece of pure gold (Au) has a volume of 50 cmWhat is its mass?
    12·1 answer
  • each chromosome originally is made of how many DNA molecules and how does this molecule appear in the chromosome
    14·1 answer
  • How does carbon dioxide affect sea urchins
    6·2 answers
  • Select all that apply.
    8·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!