1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Aleksandr [31]
2 years ago
11

6. (3 pts) After transformation, 40 colonies were present on the plate. What is the transformation efficiency of the competent c

ells if 100 ng of DNA was used for transformation of 100 ul competent cells
Biology
1 answer:
natali 33 [55]2 years ago
6 0

Answer:

Given:

Number of colonies on plate = 40

Amount of DNA used for transformation = 100ng

Volume of competent cells used = 100ul

Total volume = 1ml (1000ul)

Volume plated for growth = 200ul

The amount of cells transformed per 1 µg of DNA is called the transformation efficiency

(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA

Amount of DNA used for plating = 100ng * (200/1000)

                                                      = 100ng * (1/5)

                                                      = 100/5 = 20ng

Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng

               (2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA

Transformation efficiency = 2000 Transformants / ug DNA

                                          = 2 x 10³ Transformants / ug DNA

Explanation:

Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.

You might be interested in
The united states , canada , japan , and england are all examples of ?
Mars2501 [29]
1. developed nations
4 0
2 years ago
Turigd plant would be found in which type of environment
e-lub [12.9K]
Will be found in the environment in which it was planted in the turnda
3 0
3 years ago
What are fungus like protists
kifflom [539]

Answer:

Fungus-like protists are protists that absorb their food from dead organic matter. They are grouped into 2 groups, slime molds and water molds.

7 0
2 years ago
Need ASAP! TIA<br> Explain how probability theory can be used in agriculture.
zhuklara [117]

Answer:

occurrence

Explanation:

Probability is a mathematical construction that determines the likelihood of occurrence or nonoccurrence of events that are subjected to chance factor. Thus the farmers are interested to know the probability of occurrence of rain before and after spraying. ... In this regard probability plays a great role

4 0
2 years ago
Please help if you can!!
Darina [25.2K]

A natural change that currently affects or may affect the aquatic ecosystem of the bay of san francisco is the warming of the waters due to global warming and rising river levels.

<h3>What happens to water in global warming?</h3>

As a result of global warming and the increase in the emission of greenhouse gases, the heat retained in the atmosphere ends up being stored in the oceans, affecting the temperature and circulation of water, in addition to accelerating the process of melting of the polar ice caps.

With this information, we can conclude that  a natural change that currently affects or may affect the aquatic ecosystem of the bay of san francisco is the warming of the waters due to global warming and rising river levels.

Learn more about global warming in brainly.com/question/12908180

#SPJ1

8 0
1 year ago
Other questions:
  • The restriction enzymes of bacteria protect the bacteria from successful attack by bacteriophages, whose genomes can be degraded
    11·1 answer
  • Cells &amp; Cellular Processes
    15·1 answer
  • 1. Scientists believe that ancient ancestors of all animals were (1 point)
    6·1 answer
  • Acid rain is the direct result of acid released by burning fossil fuels.<br><br> True <br> False
    15·2 answers
  • A chemical reaction produces solid sulfur as a product. Which unit should be used to describe the mass of the sulfur produced? m
    8·1 answer
  • Which describes the process of a bacterial cell dividing to create two daughter cells
    14·2 answers
  • PLEASE ANSWER ASAPPPPP
    6·1 answer
  • Question 1
    14·1 answer
  • The group of cells that use chemicals to recycle elements in the blood are called
    10·1 answer
  • The relationship between predator and prey species is modeled here with the owls and rodents. Which statement(s) correctly evalu
    14·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!