1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Aleksandr [31]
3 years ago
11

6. (3 pts) After transformation, 40 colonies were present on the plate. What is the transformation efficiency of the competent c

ells if 100 ng of DNA was used for transformation of 100 ul competent cells
Biology
1 answer:
natali 33 [55]3 years ago
6 0

Answer:

Given:

Number of colonies on plate = 40

Amount of DNA used for transformation = 100ng

Volume of competent cells used = 100ul

Total volume = 1ml (1000ul)

Volume plated for growth = 200ul

The amount of cells transformed per 1 µg of DNA is called the transformation efficiency

(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA

Amount of DNA used for plating = 100ng * (200/1000)

                                                      = 100ng * (1/5)

                                                      = 100/5 = 20ng

Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng

               (2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA

Transformation efficiency = 2000 Transformants / ug DNA

                                          = 2 x 10³ Transformants / ug DNA

Explanation:

Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.

You might be interested in
What structure encloses almost all bacteria? Do bacteria have a structure that encloses the genetic material?
Xelga [282]
First question - cell well
second question - bacteria do not have a membrane enclosed nucleus. the and of bacterial cells is found loose in the cytoplasm.
7 0
3 years ago
This holds an organisms hereditary information.
Luba_88 [7]

Answer:

Deoxyribonucleic acid (DNA) hold the hereditary material that acts as a blueprint for the development and functioning of life process on the earth. It is a blueprint for every cell in every organism.

Its major function is to encode the DNA sequence of amino acid by using the triplet genetic code that code for a specific protein that perform a specific function in the body that are necessary for the normal function of the life processes.







4 0
3 years ago
Read 2 more answers
Below is the double-stranded DNA sequence of part of a hypothetical yeast genome. Within this sequence is a small gene, PICO8, t
Serjik [45]

Answer:

D. There isn't enough information to determine which is the coding strand

Explanation:

In this protein, it would be necessary to observe a start codon (ATG) and one-stop codon (either TAA, TAG, or TGA) which should be separated by six (6) intern codons. It is not observable this nucleotide sequence in any of both DNA strands.

6 0
3 years ago
Natural selection ___.
Afina-wow [57]
The answer is A) prepares organisms for future changes in the environment
6 0
3 years ago
Sentence with electron transport chain?
sukhopar [10]
The electron transport chain is the final stage of aerobic respiration leading to the forming of ATP in the inner membrane of the mitochondrion<span>. 

</span>Mitochondrion<span>, is a membrane-bound organelle found in the cytoplasm of almost all eukaryotic cells. 
</span>
<span>An eukaryote is any organism whose cells contain a nucleus and any other organelles within the membranes.</span>
6 0
3 years ago
Other questions:
  • What is responsible for determining which direction water will diffuse across the plasma membrane of cells?
    9·1 answer
  • Which process allows a mammal to continue to grow in size
    15·1 answer
  • If a plants cells cannot fight a pathogen, what will the cells do?
    9·2 answers
  • Need some help please
    9·1 answer
  • What is convection in the atmosphere called ? please answer quick !!
    14·2 answers
  • How the solar enegy enters inside the cell
    12·1 answer
  • What might happen to the food web below if the number of phytoplankton drastically decreased? The small fish and wading birds wo
    12·2 answers
  • During active transport, what is required to move material across the cell membrane? А energy B water С oxygen D cytoplasm​
    14·1 answer
  • AHAHAAH im down bad Hey lol
    9·2 answers
  • Why do humans have a strong impact on the fast carbon cycle?
    15·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!