A biologist wishes to take a useful gene found in pine trees and introduce it into some bacterial cells. Her experimental proced
ure is given below. 1. Use a restriction enzyme to cut a gene out of the pine tree DNA.
2. Use a restriction enzyme to cut open a plasmid.
3. Treat the plasmid with DNA ligase.
4. Mix the desired gene and plasmid together.
5. Introduce the plasmid into bacterial cells.
Which steps should be switched in the biologist's procedure?
In Recombinant DNA technology which involves DNA molecules formed by recombination to bring together genetic material from different sources, producing sequences that would normally not be found in the genome. Some basic steps are to be followed in the formation of your recombinant DNA. The restriction enzyme used in cutting the gene of interest from the pine tree DNA should also be the same used in cutting open the plasmid to make complementary sticky ends. Then integration of the isolated gene of interest into the cut plasmid and then this is treated with ligase to join the cut sticky ends. This construct is then introduced into bacterial cells.
When Surita sees a man walking around the shopping mall in December and notices that he is very robust, has a long white beard, and wears thin rimmed eyeglasses, she immediately assumes that he is a Santa Claus actor taking a break; she is engaging in a(n) __AUTOMATIC_______ process.
The movement is caused by the convection currents that roll over in the upper zone of the mantle. This movement in the mantle causes the plates to move slowly across the surface of the Earth.
The accumulation of several mutations in the main gene on the virus's surface may be able to give H7N9 the ability to spread like human flu viruses do, passing from person to person through coughing and sneezing.
countries with higher populations are likely to use more resources
Explanation:
The vastness of the threats to the ecosystem is directly linked to human population size and resource uses per person. Resource use, waste production and environmental degradation are accelerated by population growth.
