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Novosadov [1.4K]
4 years ago
12

If a scientists data was contaminated what is the best course of action

Biology
1 answer:
Varvara68 [4.7K]4 years ago
3 0

Answer:

  • Of course, the best course of action is to <u>re-analyze</u> the samples again and generate a new dataset. This would be time consuming and may require further cost. But this would be the best possibility to eliminate bias from the data.
  • In case, if re-analysis is not possible, then another option is to see the standard deviation among replicate. If only one replicate is contaminated, and other two are fine, then we can simply remove the contaminated sample considering it as an outlier.

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3 years ago
If an enzyme's active site contains only two ionizable residues, an arginine and a glutamate (pKa's of 2.9 and 9.1, respectively
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Answer:

Optimum pH= 6

Optimum pH for the given enzyme would be the average of pKa values of two amino acids (2.9 + 9.1 /2= 6)

Explanation:

The pKa represents the association constant of amino acids. It is calculated by taking the negative log value of the ratio of a dissociated acid and the conjugated base which in turn regulates the isoelectric point (pI) of the protein.

The amino acids present in the active site of the enzyme regulate the interaction of enzyme and substrate. The given enzyme contains two ionizable amino acids with pKa values of 2.9 and 9.1 respectively. Therefore, the optimum pH of the given enzyme would be 2.9 + 9.1 / 2 = 12/2 = 6.  

At pH 6, both the ionizable amino acids would serve as a good buffer with their active proton donor and proton acceptor species.

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