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Usimov [2.4K]
3 years ago
15

What are the top challenges for Hollywood managers? Can you think of other industry segments that face similar problems?

Medicine
1 answer:
aleksandr82 [10.1K]3 years ago
5 0
<h3><u> Top challenges for Hollywood managers:</u></h3>

Challenges that face Hollywood managers include finding the right film executives who will ensure the success of the production and the company. Another challenge is finding the right cast that will relate to the public which will in turn increase the company’s profit.

There is also a challenge of making stories that will bring in audience and generate revenue over time. Data mining is very important in Hollywood to analyse the success and failure of the company. Other industries which face similar problems include governments, technology companies, etc.

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Answer:

Okay

Explanation:

Human topoisomerase I plays an important role in removing positive DNA supercoils that accumulate ahead of replication forks. It also is the target for camptothecin-based anticancer drugs that act by increasing levels of topoisomerase I-mediated DNA scission. Evidence suggests that cleavage events most likely to generate permanent genomic damage are those that occur ahead of DNA tracking systems. Therefore, it is important to characterize the ability of topoisomerase I to cleave positively supercoiled DNA. Results confirm that the human enzyme maintains higher levels of cleavage with positively as opposed to negatively supercoiled substrates in the absence or presence of anticancer drugs. Enhanced drug efficacy on positively supercoiled DNA is due primarily to an increase in baseline levels of cleavage. Sites of topoisomerase I-mediated DNA cleavage do not appear to be affected by supercoil geometry. However, rates of ligation are slower with positively supercoiled substrates. Finally, intercalators enhance topoisomerase I-mediated cleavage of negatively supercoiled substrates but not positively supercoiled or linear DNA. We suggest that these compounds act by altering the perceived topological state of the double helix, making underwound DNA appear to be overwound to the enzyme, and propose that these compounds be referred to as ‘topological poisons of topoisomerase I’

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