Answer:
False
Explanation:
This might not even be considered stealing if the person removing the cologne puts it back but it is definitely not considered abuse which is where someone verbally or physically does harm to the victim.
The <em><u>MESOSPHERE </u></em>is the coldest layer and the hottest layer is <u><em>THERMOSPHERE </em></u> is the hottest layer in the atmosphere.
Answer: Nutrients and Sunlight
Explanation:
Nutrients and sunlight are two important factors for photosynthesis. Nutrients are necessary for keeping the plant alive these nutrients such as inorganic minerals are obtained by plants through soil. The sunlight is essential for splitting the molecules of water so as to produce oxygen as a product along with carbohydrates.
Answer:
b
Explanation:
nitrogen will slowly go down due to not having enough organic matter
Answer:
The options A, B, and D are all valid.
Explanation:
- The reason is that some proteins require molecular chaperones if they are to fold properly within the environment of the cell. In the absence of chaperones, a partially folded polypeptide chain has exposed amino acids that can form non-covalent bonds with other regions of the protein itself and with other proteins, thus causing nonspecific aggregation of proteins.
- The option A) is correct because the protein you are expressing in bacteria is being made in large quantities, it is possible that there are not enough chaperone molecules in the bacterium to fold the protein. Expressing the protein at lower levels might increase the amount of properly folded protein.
- The option B) is correct as urea should solubilize the protein and completely unfold it. Removing the urea slowly and gradually often allows the protein to refold. Presumably, under less crowded conditions, the protein should be able to refold into its proper conformation.
- The option C) is not correct as treating the aggregate with a protease, which cleaves peptide bonds, will probably solubilize the protein by trimming it into pieces that do not interact as strongly with one another; however, chopping up the protein will also destroy its enzymatic activity.
- The option D) is correct because overexpressing chaperone proteins might increase the amount of properly folded protein.
- The option E) is not correct as heating can lead to the partial denaturation and aggregation of proteins to form a solid gelatinous mass, as when cooking an egg white, and rarely helps solubilize proteins.
