Answer:
Carbohydrate and mitochondria are closely connected with each because carbohydrate is a food substance from which energy is extracted by the mitochondria of the cell. Mitochondria is called the power house of the cell. Its main function is to make energy for the cell which used this energy to perform various function by using food material such as carbohydrate. So we can say that both are connected with each other.
Answer:
First, you need to retrieve the FASTA sequences of the already sequenced genes. After that, you should make a sequence alignment on a software like CLUSTALW (https://www.ebi.ac.uk/Tools/msa/clustalw2/) to seek for the conserved regions of the gene. Based on this regions, you will design your FISH probe, which for bacteria can start in 33 bp (Ming Tan <em>et al.</em>, 2019), but a 200 bp probe is a very suitable size. There are several companies that fabricates this custom probes, and sell all the reagents to perform the experiments. Having this, you will perform your FISH experiment to see which cells are actively degrading Toluene.
Explanation:
