Question

the appropriate negative control the researchers most likely used when measuring the reaction rate in the presence of each organism’s enolase.

Answer 1

Answer:

In a negative control, one would expect to get negative results indicative that the results obtained were due to the experimental conditions and without the experimental conditions, the expected results would not be obtained. Hence a suitable negative control would be to not include any enzyme used in the actual experiment but include the substrates of the reaction to ensure that the enolase does not catalyse the same reaction in the presence of the substrates.

Answer 2

Answer:

Negative control group is Chloroflexus aurantiacus bacteria

Explanation:

Complete Question

Enolase is an enzyme that catalyzes one reaction in glycolysis in all organisms that carry out this process.  The amino acid sequence of enolase is similar but not identical in the organisms. Researchers purified  enolase from Saccharomyces cerevisiae, a single-celled eukaryotic yeast that grows best at 37°C, and  from Chloroflexus aurantiacus, a bacterium that grows best at the much higher temperature of 55°C. The researchers compared the activity of purified enolase from the two organisms by measuring the  rate of the reaction in the presence of varying concentrations of substrate and a constant amount of  each enzyme at both 37°C and 55°C.

(b) Identify the appropriate negative control the researchers most likely used when measuring the  reaction rate in the presence of each organism’s enolase.

Solution

In a well-designed experiment, a positive control group is the one which receives the treatment and is expected to show some response against the treatment

However, a negative control group is the one which receives a treatment but no response is expected.  

In the given experiment-  

Positive control group is Saccharomyces cerevisiae, yeast that grows best at 37°C  

Negative control group is Chloroflexus aurantiacus, a bacterium that grows best at a temperature higher than that of 55°C but still is treated at 55°C