Definition
A technique which is used to separate, DNA, RNA or protein pieces from each other under the influence of electric field on the basis of their molecular size is known as gel electrophoresis.
Explanation
This method is very reliable for separation of large size molecule (over 1 million Da). Materials which are required for gel electrophoresis include:
1. TAE stock buffer
2. 1% agrose gel
3. Nucleic acid loading dye
4. Ethidium bromide
Procedure:
First prepare a stock solution of TAE buffer by adding appropriate amount of TAE in distilled water. then prepare 1% agrose by adding 1X TAE, some amount of agrose in water and heating it in microwve oven to mix them will. then pour agrose gel on tray and fix comb in it and keep it untill agrose dry. Then remove comb and pour some quantity of nucleic acid along with loading dye and ethidium bromide in each well. EtBr is used for staining nucleic acid.
When sample is poured in all well also pour reference marker in one well for comparison. now connect it with voltage for 30-35 min. After this take gel and see it under UV. a large number of nucleic acid pieces will be seen on gel under UV. those pieces which have small molecular weight will cover more distance compared to those having larger molecular weight.
the answer is c: because when we start logging animals lose the areas were they resided
If you are checking a cell for a zero value and the cell is blank, the test evaluates to true. For example, if you have the following formula in cell A1 =IF(B1=0,"zero","blank") and B1 is blank, the formula returns "zero" and not "blank" as expected.
If the range might contain a blank cell, you should use the ISBLANK function to test for a zero value, as in the following example: =IF(ISBLANK(B2),"blank",IF(B2=0,"zero","other")) Note that the above formula returns "zero" if there is a zero value in the cell, "blank" if the cell is blank, and "other" if anything else is in the cell.
You must always use the ISBLANK formula first before you test for a zero value. Otherwise you will always return a "true" for the zero value, and never get to the test for the ISBLANK formula.
Iron, oxygen and water react with the iron creating a substance known to us as rust.
Hope this helps.
Elaborative rehearsal is a memory system that includes contemplating the significance of the term to be recalled, instead of essentially rehashing the word to yourself again and again. For instance, you have to recall the expression "neuron."
