In a well-designed experiment, there will only be one factor tested at
any one time. If there are more factors than this being tested, the
results can become quite convoluted, less focused and more difficult to
understand. Although it might seem that the more you test the more you
know, this is not the best way to obtain accurate results.
At high temperatures the shape of the enzyme is altered so that it is no longer complementary to its specific substrate. This effect can be permanent and irreversible and is called denaturation
