A hypothesis is when you make an educated guess before you complete the experiment. It is basically an inference on what might happen next. So a conclusion made based on facts.
Answer:
She left Ligase enzyme out of the mixture.
Explanation:
DNA replication is the process of formation of new molecules of DNA from the existing one. This process occurs in semiconservative way i.e each new strand consist of one newly synthesised strand and one old parent strand that act as template for the formation of new strand. replication on one strand occurs continuously from 5 prime to 3 prime but on other strands it occurs discontinuously from 5 prime to 3 prime because DNA polymerase moves only in one direction from 5 prime to 3 prime. Hence in discontinuous strands gaps remained. These gaps are filled by Ligase enzyme.
Requirements for DNA replication:
DNA replications needs
Primers
DNA Polymerase enzyme
DNA helicase enzyme
Single nucleotide binding protein
dNTPs
and Ligase enzyme.
Correct choice:
She left Ligase enzyme out of the mixture.

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Read more: <span>http://www.scienceclarified.com/Al-As/Antibody-and-Antigen.html#ixzz4YlY105DD</span></span>