Question

In the presence of a non-competitive inhibitor a Lineweaver-Burk plot has a higher y-intercept than a control (without any inhibitor), and the x intercept is the same as that for the control. Explain what this indicates.

Answer 1

Answer:

The Lineweaver- Burk plot is representation of Lineweaver-Burk equation of enzyme kinetics in graphical plot that is presented by the Hans Lineweaver and Dean Burk.

In the presence of a non competitive inhibitor shows higher y-intercept than a control in absence of any inhibitor whereas the x intercept is the same as the control that suggests or tells that maximum enzyme activity of the reaction is decreased, Higher Y intercept than controls means that it is same as 1/Vmax. The X intercept indicates that there is no effect on Km of the enzyme reaction.