The ischium bone forms the superior part of the pelvic girdle.
<h3>What is the structure of the pelvic girdle?</h3>
In the bottom region of the trunk, there is a bony structure known as the pelvic girdle that resembles a ring. It joins the lower limbs to the axial skeleton. There are two types of pelvises: the bigger pelvic and the lesser pelvis.
The pelvis is made up of two paired hipbones that are joined at the pubic symphysis in front and by the sacrum in back. Each hipbone is composed of three bones: the blade-shaped ilium above and to either side, which determines the hips' width; the ischium below, on which the weight is placed when sitting; and the pubis in front. Early in maturity, all three come together at a triangle suture in the acetabulum, the cup-shaped socket that connects to the head of the femur to create the hip joint.
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Answer:
if the promoterless lacZ is inserted close to any gene(s) or operon, then the laz gene will also be transcribed when the operon gets expressed. The polycystronic RNA will eventually produce a beta-galactosidase protein(product of lacZ gene) which will cleave X-Gal( structural analog to lactose), which will produce blue chromogenic substance.
prepare LB agar. Allow it to cool, add X-gal before it solidifies, pour to plates.
create grids and patch plate each strain on it. Just pick the strain that looks blue, amplify the sequence upstream of the lacZ and sequence it.
Explanation:
Just pick the strain that looks blue, amplify the sequence upstream of the lacZ, and sequence it.
