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krek1111 [17]
3 years ago
11

Arrange the steps of extraction of DNA in the order in which theyshould be performed.

Law
1 answer:
frozen [14]3 years ago
4 0

Step 1: Lysis

In this step, the cell and the nucleus are broken open to release the DNA inside and there are two ways to do this. First, mechanical disruption breaks open the cells. This can be done with a tissue homogenizer (like a small blender), with a mortar and pestle, or by cutting the tissue into small pieces. Mechanical disruption is particularly important when using plant cells because they have a tough cell wall. Second, lysis uses detergents and enzymes such as Proteinase K to free the DNA and dissolve cellular proteins.

Step 2: Precipitation

When you complete the lysis step, the DNA has been freed from the nucleus, but it is now mixed with mashed up cell parts. Precipitation separates DNA from this cellular debris. First, Na + ions (sodium) neutralize the negative charges on the DNA molecules, which makes them more stable and less water-soluble. Next, alcohol (such as ethanol or isopropanol) is added and causes the DNA to precipitate out of the aqueous solution because it is not soluble in alcohol.

Step 3: Purification

Now that DNA has been separated from the aqueous phase, it can be rinsed with alcohol to remove any remaining unwanted material and cellular debris. At this point, the purified DNA is usually re-dissolved in water for easy handling and storage.

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Answer:

The correct answer is A. Decided during the Constitutional Convention of 1787, this accommodation onrepresentation in the proposed US House of Representatives tacitly acknowledged slavery and kept the Southern slave states from rejecting the Constitution. It was called the Three-Fifths Compromise.

Explanation:

The Three-Fifths Compromise was a compromise reached between delegates from the southern states and delegates from the northern states during the Constitutional Convention in 1787. The debate centered on on the fact whether slaves would be counted at the same time as determining the total population of a state to determine legislative representation and for taxative functions. The matter was important, while that population number then used to determine how many seats the state would have in the House of Representatives for the next ten years. The effect was to give the southern states one-third more seats in Congress and one-third more votes they would otherwise have, allowing slave interests to largely dominate the United States government until 1865.

3 0
3 years ago
Briefly explain peremptory challenges?​
timurjin [86]
A peremptory challenge is used by attorneys in the jury selection process to excuse potential jurors without providing a reason why. In this lesson you will learn about the use of peremptory strikes, as well as the laws designed to protect the integrity of the process.
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3 years ago
Discuss the role of judicial review in relation to administrative agencies.
KATRIN_1 [288]

Answer: First, courts will examine the statutory authority for an agency's action and will invalidate agency choices that exceed these limits. In addition, a court may examine an agency's discretionary decisions, or discrete actions with legal consequences for the public.

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3 years ago
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Answer:

The answer is C. Legislative

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2 years ago
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Answer:

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