Question

Fluorescence recovery after photobleaching (FRAP) is used to monitor the movement of fluorescently labeled molecules within the plane of a cell membrane. The molecules labeled are often proteins, but lipids can be labeled too.How would the curve that represents FRAP for labeled proteins compare to the curve representing labeled lipids?Choose one:A.The FRAP curve for lipids would show a much more rapid recovery but only reach about 50% of the initial levels of fluorescence.B.The FRAP curve for proteins would show a much more rapid recovery but only reach about 50% of the initial levels of fluorescence.C.The FRAP curve for proteins would show a much more rapid recovery to initial levels of fluorescence.The curves would be identical.D.The FRAP curve for lipids would show a much more rapid recovery to initial levels of fluorescence

Answer 1

Answer:

D. The FRAP curve for lipids would show a much more rapid recovery to initial levels of fluorescence

Explanation:

FRAP means Fluorescence Recovery After Photobleaching. FRAP helps to determine the way in which lipids and proteins interact with one another in the cellular membrane. It also helps to determine how lipids and proteins diffuses or move freely both in the cell membrane.

Lipids have the ability to move faster than proteins, therefore, recovery of fluorescence for labeled lipids would be very fast and would be able to reach the initial level of fluorescence very quickly.

Answer 2

Answer: The correct answer is D; The FRAP curve for lipids show a much more rapid recovery to initial levels of fluorescence.

Explanation:

FRAP (Fluorescence recovery after photobleaching) is utilized to portray the mobility of cellular molecules. The trial arrangement includes a microscope, a light source and a fluorescent probe coupled to the molecule of intrigue. A few images utilizing a low light level are obtained to determine the initial fluorescence, and afterward an elevated level of light for a brief timeframe inside an area of intrigue is applied to bleach the fluorescence. Then finally, another arrangement of images utilizing a light level adequately low to prevent further bleaching is obtained to pick up understanding into the redistribution of molecules through recovery of fluorescence.

The FRAP technique was first used to dissect the versatility of individual lipid particles inside a cell membrane. FRAP can likewise be utilized to examine protein elements outside the membrane.

The FRAP curve of lipids will show a much more rapid recovery to initial levels of fluorescence because lipids have the tendency to move much faster than protein, hence recuperation of fluorescence for labelled lipids would be considerably more fast and should arrive at the initial level of fluorescence decently fast.