Answer:
The smallest size tube that will hold the entire reaction is 0.5ml
Explanation:
25ul (DNA sample) + 25ul (enzyme) + 50ul (buffer) + 400ul (water) = 500ul total
since 1000ul=1ml, then 500ul = 0.5ml
Answer:
The device that mostlikely would not be used P20
Explanation:
P20 is a pipette used to measure and sample volumes of 20ul maximum.
Answer:
The reaction mix will have 400 units of the enzyme.
The working dilution will be 0.8 Units/ul.
Explanation:
The mix has 20ul of the enzyme which is 20Units/ul. Then we have 20ulx20Units/ul= 400 Units of enzyme in the mix.
Since the final volume is 500ul, the final concentration 400Units/500ul=0.8 Units/ul.
Answer:
Explanation:
The light reactions of photosynthesis involve light-driven electron and proton transfers, which occur in the thylakoid membrane, whereas the dark reactions involve the fixation of CO2 into carbohydrate, via the Calvin–Benson cycle, which occurs in the stroma (Figure 3).
Answer:
The correct answer is option D. "Use of siRNA to the kinase mRNA in question".
Explanation:
Small interfering RNA (siRNA), also known as interfering or silencing RNA, is a small molecule of RNA (20-25 base pairs) that binds to a particular region of a messenger RNA, avoiding its translation into protein. These type of interfering molecules are often used to silence genes and corroborate their function by analyzing what are the biological consequences of avoiding their translation into protein. Therefore, if a scientist wants to know if a kinase is indeed responsible for the decrease in signaling, a good option is to use a siRNA to the kinase mRNA in question.
The correct answer is B. Each of the claimants or their living children.
Explanation:
One of the basic principles in genetics establishes genes are passed from the parents to their offspring. Indeed, children receive 50% of genes from each parent. This implies the daughter of the tsar and tsarina will have share part of the DNA including mtDNA (mitochondrial DNA) of her mother, father, and siblings.
According to this, to prove if someone is Anastasia or not, it is possible to use the mtDNA sample from the tsar, tsarina, or death children but not from the claimants. This is because the claimants are not confirmed relatives of the tsar or the tsarina, and therefore their DNA cannot be used to determine if a woman is the daughter of the tsar and tsarina. Indeed, a woman claiming to be Anastasia can share DNA with another claimant and this does not prove they are related to the tsar and tsarina or that the woman is the real Anastasia.
The process is fertilisation. Hope this helps you.
