Technically the are both right and wrong. Solid substances are most likely to sublime is they have... high vapor pressure and weak intermolecular forces.
Answer:
I would expect to extract the acetic acid.
Explanation:
In the first step, since we are adding a concentrated acid,<u> it will react with the bases present in the mixture (diethylamine and ammonia) </u><u>forming salts</u><u>, </u><u>which are soluble in water</u>. Therefore, after draining the aqueous layer, we will have phenol and acetic acid left in the organic layer.
In the second step, we are adding a diluted base, so it will react with a strong acid. This compound is acetic acid, and its salt will be present in the aqueous layer. Phenol will be left on the organic layer.
We can classify the ion molecules as follows:
PO4^3- = Bronsted Base
ClO2- = Bronsted Base
NH4+ = Bronsted Acid
HCO3- = Both
H2PO4- = Both
For a Bronsted-Lowry acid and bases, an acid is a proton (hydrogen ion) donor. A base is a proton (hydrogen ion) acceptor. Hope this answers the question. Have a nice day.
Answer:
64,433.6 Joules
Explanation:
<u>We are given</u>;
- Volume of water as 220 mL
- Initial temperature as 30°C
- Final temperature as 100°C
- Specific heat capacity of water as 4.184 J/g°C
We are required to calculate the amount of heat required to raise the temperature.
- We know that amount of heat is calculated by;
Q = mcΔT , where m is the mass, c is the specific heat, ΔT is the change in temperature.
Density of water is 1 g/mL
Thus, mass of water is 220 g
ΔT = 100°C - 30°C
= 70°C
Therefore;
Amount of heat, Q = 220g × 4.184 J/g°C × 70°C
= 64,433.6 Joules
Thus, the amount of heat required to raise the temperature of water is 64,433.6 Joules
Answer:
High concentration of glutathione should be included in the elution buffer for the given experiment to remove protein from the column.
Explanation:
Affinity chromatography is one of important biochemical technique of chromatography which depends on the affinity of ligand for the receptor.Here the ligand is a protein mixture which act as mobile phase and the receptor is present in the wall of chromatography column act as stationary phase.When the protein mixture is applied on the top of the column the substances present within the protein that have high affinity for the receptor present in the walls of chromatography column binds to the later but rest of the protein pass away through the column.High concentration of ligand is used within an buffer solution to remove the desired protein from the column.
From this point of view it can be stated that in the given question high concentration of glutathione should be used to remove the desired protein from the column.
