Answer:
The correct answer will be thiamine pyrophosphate (TPP) → dihydrolipoamide → Flavin adenine dinucleotide (FAD).
Explanation:
The pyruvate dehydrogenase complex is a multi-functional enzyme which decarboxylates the pyruvate to form Acetyl CoA. The complex consists of three catalytic enzymes which attach the non-protein part to its structure.
The prosthetic groups are the non-protein groups which bound strongly to the enzyme. In this complex the prothetic groups are :
1. Thiamine pyrophosphate (TPP)- attached to Pyruvate dehydrogenase (E1).
2. Lipoamide: attached to dihydrolipoyl transacetylase (E2).
3. Flavin adenine dinucleotide (FAD): attached to dihydrolipoyl dehydrogenase (E3).
These enzymes work in sequence: E1 →E2→ E3 therefore, the correct order of prosthetic group will be thiamine pyrophosphate (TPP) → dihydrolipoamide → Flavin adenine dinucleotide (FAD). Thus, this is the correct order.
Answer:
Enzymes are required to catalyse the reactions in both stages
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