Answer:
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Seymour Benzer used phage T4 mutants, Experimental
evidence that a gene is a linear sequence of nucleotide pairs. Some regions of
chromosomes have "hot spots" for mutations. Benzer's experimental
approach to fine structure mapping of the rII locus. Generated 1612 spontaneous point mutations
and several deletions in rII locus identified two complementation groups, rIIA
and rIIB. Mapped locations of deletions
relative to each other using recombination.
Mapped locations of point mutations relative to the deletions. Tested
for recombination between all point mutations within the same complementation
group.
Viruses are not considered living because they cannot survive without a host organism, and they do not exhibit the characteristics of life.
Answer:
It is pertinent to understand what hypertonic, hypotonic, and isotonic solution means before setting out to explain how a cell reaches equilibrium in each type of solution.
A hypertonic solution is one whose solute concentration is higher than that of the sap of a cell that is immersed in it.
A hypotonic solution is one with the same solute concentration as that of the sap of the cell immersed in it.
An isotonic solution has a lower solute concentration than that of the sap of the cell immersed in it.
In biological systems, water molecules move by osmosis from the region of higher water potential or lower concentration of solutes to the region of lower water potential or higher concentration of solute. An equilibrium is reached when there is no net movement of water between two sides. Hence;
A cell placed in a hypertonic solution will lose water to the surrounding solution until an equilibrium is reached. This means that such a cell will end up shrinking (wilting) or even dying due to loss of water from the cell sap.
A cell placed in a hypotonic solution will gain water from the surrounding solution until there is no net movement of water anymore. Such a cell might become turgid or even burst out its cell content.
A cell placed in an isotonic solution will neither gain nor lose water because the cell sap and the surrounding solution have equal solute concentrations.
Explanation: