Answer:
Polymerase chain reaction (PCR) test is used to duplicate traces of DNA from any living form, for identification purposes. The correct sequence is 1, 3, 2; based on the options given.
Explanation:
The steps to be followed for the PCR procedure are:
<em>Denaturation</em>
Option 1. Involves incubation at 94°C to denature DNA strands into single strands by breaking of weak hydrogen links
<em>Annealing</em>
Option 3. Temperature is lowered to 60°C to allow primers joining their similar DNA sequences.
<em>Elongation</em>
Option 2. Incubate at 72°C to promote the extension of the DNA strands, actually extending them.
I believe it is Mechanical Energy
Answer:
All the crossing will be like this after realizing it:
Explanation:
Gl x Gl
GG, Gl, Gl, ll
gl x gl
gg, Lg, Lg, LL
On the first crossing we'll have 50% Gl and both other genotypes will be 25%
On the second crossing we'll have 50% Lg and both other genotypes will be 25%.
The sodium amytal test involves the injection of a small amount of sodium amytal into the carotid artery on one side of the neck. This injection anesthetizes the hemisphere on that side for a few minutes.
A method that involves injecting a small amount of a barbiturate into the carotid artery on one side of the head to assess hemispheric functions, usually memory and language. The cerebral hemisphere that was injected selectively becomes impaired for 10 to 15 minutes during this operation.
Various cognitive tests are given while each hemisphere is seperately anaesthetized; deficiencies on these tasks imply that these functions are represented in the anaesthetized hemisphere. Prior to a temporal lobectomy, the Wada test may be utilised in cases with severe and uncontrollable epilepsy. Also known as the Wada technique, intracarotid sodium Amytal test (ISA), Wada dominance test, and intracarotid amobarbital procedure.
Learn more about sodium Amytal test (ISA) here;
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