ATP is not generated directly in the citric acid cycle. Instead, an intermediate is first generated by substrate-level phosphorylation. The intermediate is GTP.
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What is GTP?</h3>
- A purine nucleoside triphosphate is guanosine-5'-triphosphate.
- It serves as one of the components necessary for the creation of RNA during transcription.
- The main distinction between its structure and that of the guanosine nucleoside is the presence of phosphates on the ribose sugar of nucleotides like GTP.
- Also known as guanosine triphosphate, this energy-dense nucleotide is similar to ATP and is made up of guanine, ribose, and three phosphate groups.
- It is required for the creation of peptide bonds during protein synthesis.
- Adenine nitrogenous base, sugar ribose, and triphosphate make up ATP, a nucleoside triphosphate, whereas guanine nitrogenous base, sugar ribose, and triphosphate make up GTP.
- This is the main distinction between the two compounds.
- The alpha-guanosine subunit's diphosphate (GDP) is converted into guanosine triphosphate (GTP), and the GTP-bound alpha-subunit subsequently separates from the beta- and gamma-subunits.
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Probably not. some bacteria produce enzymes that break down hemoglobins in RBC.
Answer:
Level 1...Plants and algae make their own food and Plants and algae make their own food and are called producers.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
