Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Explanation:
(1) The conglomerate contains some nonsedimentary rock fragments. (2) The conglomerate was formed from material that was buried deep underground. (3) The conglomerate's pebbles are all weathering at the same rate.
Answer:
Explanation:
Cerebellum is a leaf-like structure that is a part of the hind brain and is responsible for:
- coordinating skeletal muscle movements.
- controls movements, posture and balance.
- involved in learning too.
So, if the cerebellum of the brain is affected/injured, a person can have difficulty in keeping their body in a balanced position, coordinate voluntary movements of skeletal muscles and learning.
![\rule[225]{225}{2}](https://tex.z-dn.net/?f=%5Crule%5B225%5D%7B225%7D%7B2%7D)
Hope this helped!
<h3>~AH1807</h3>
It is important to understand because it's the way all cells reproduce (except sperm and egg cells, they do meiosis), and our cells are the essential parts of our whole being.
