To identify the host cell that has taken up the gene of interest
The approximate LD50 of the least toxic substance will be 130 mg/Kg.
- The dose necessary to cause the death of 50% of a population under test after a predetermined test period is known as the LD50 value for a drug.
- Commonly used as a broad indicator of a substance's acute toxicity are LD50 values.
- Greater toxicity is indicated by a lower LD50. A low LD50 of 500 mg/kg or less implies severe toxicity.
- Moderate toxicity is indicated by an LD50 of 500 to 1,000 mg/kg. A modest toxicity level of 1,000 to 2,000 mg/kg is indicated.
- The species is especially vulnerable to the poison if the LD50 is low. The lethal dose (LD50) of a specific poison might change significantly between several animal species.
- Thus out of all the options, LD50 130 mg/kg will have the lowest toxicity and 20 mg/kg will be the highest.
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Answer:
2. lluvia, sequía, derretimiento de glaciares, aumento de la temporada etc
seed coat protects the cotyledons and embryo from outer environment and from attack of pathogens
pollen grain has a sculptors covering with weak points(germ pores) in it through which pollen tube comes out.
A spore is generally protected by 2-3 tough resistant walls around to tied over the unfavourable conditions.
