Answer:
A) A transgene integrated into a heterochromatic region of the genome.
Explanation:
This is most likely the reason why the transgene is not expressed in the fifth cell line. It is likely that the transgene was integrated into a heterochromatic region of the genome. Heterochromatin consists of genetically inactive satellite sequences, and many of these are repressed to various extents. Moreover, because this is a tightly packed form of DNA or condensed DNA, it is inaccesible to polymerases and thus not transcribed.
Because only the chromosomes can be seen in a karyotype, and microdeletions or insertions are mutations at the molecular level, it is virtually impossible to detect such mutations at the chromosomal level.
These are the things that convert nitrogen in the soil -cyanobacteria<span>participate. After nitrogen has been fixed, other </span>bacteria<span> convert it into </span>nitrate<span>, in a process known as nitrification.</span>
