When calibrating a spectrophotometer, measuring absorbance concurrently is the best option for a blank since it is proportional to the concentration.
Beer's law states that A = a b c, wherein there is the diffusion coefficient at a constant, b is the actual route length, & c is the concentration. Direct proportionality exists between b and c and absorbance.
Once the route length is doubled, incident light contacts double as many molecules in the solution. The consequence is a doubling of absorbance, which is equivalent to a doubling of molecule concentration.
There are two ways to detect chemicals using spectrum scanning. One approach involves turning the monochromator continuously with a stepping motor while gradually altering the wavelength connected to the output slit.
It is more practical to use diode array detectors. Up to a few hundred photodiodes may be incorporated into the chip that makes up this device.
Learn more about spectrophotometers at
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Answer:
Radioactive labeling is a procedure used to monitor the path followed by a chemical element within a biological system to demonstrate the source.
Hypothesis:
The free oxygen produced during photosynthesis comes from water.
Procedure.
Single-celled algae were placed in four petri dishes containing carbon dioxide, glucose dissolved in Water.
In each box the oxygen was radioactively marked.
1 petri dish with CO2 marked. Experimental groups
2 petri dish with H2O marked. Experimental groups
3 petri dish with Glucose marked. Experimental groups (control)
4 petri dish with CO2 + H2O + Glucose, all marked. (control).
Increases
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The limbic system is responsible for controlling learning and emotional behavior
